The news conference was held at the grandeur Guildhall, a grade one listed building dating back to the 15th century.
Olympic gold medallist Joshua has the opportunity to join legends Muhammad Ali, Evander Holyfield, Lennox Lewis and Vitali Klitschko as a three-time champion.
He was asked about the confrontation in June when he felt disrespected by Dubois and threatened to throw a chair across the champion’s face.
“You should never let anyone take an inch because they’ll end up taking a mile,” replied Joshua. “You know what I mean Dan?”
But Dubois, vacantly, replied: “Sorry?”
The Londoner did, though, speak to address rumours surrounding his trainer Don Charles, who has not yet been seen in fight week.
“As long as he’s in my corner on fight night, I’m alright,” added Dubois. “Everything is good.”
Joshua lost his belts to Usyk in 2021 and was defeated by the Ukrainian in the rematch the following year. He has since worked his way back to the mandatory challenger position with four dominant wins.
Joshua, who will headline a British stadium for a sixth time, said he has been looking at some of his past fights in the country as motivation.
“It’s good to be back,” he added. “I’m ready to rumble and remind everyone what I’m capable of.”