In the last two years, the force said it had received 509 calls relating to anti-social behaviour involving e-scooters, and 441 reports of them being ridden dangerously.
As part of a new approach, police have moved to a “seize first” position with e-scooters, with no requirement for officers to issue an initial warning at the time of a first offence.
Officers have been working with various partners, including CCTV operators from Derby City Council, to intercept illegal riders.
During the operation on 29 November, e-bikes were tested by officers to check if they met legal requirements to be used on public roads, and if not, the e-bike was seized.